Variation in the molecular weight of Photobacterium damselae subsp. piscicida antigens when cultured under different conditions in vitro
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Variation in the molecular weight of Photobacterium damselae subsp. piscicida antigens when cultured under different conditions in vitro
The antigenicity of Photobacterium damselae (Ph. d.) subsp. piscicida, cultured in four different growth media [tryptone soya broth (TSB), glucose-rich medium (GRM), iron-depleted TSB (TSB + IR(-)), and iron-depleted GRM (GRM + IR(-))] was compared by enzyme-linked immunosorbent assay (ELISA) and Western blot analysis using sera obtained from sea bass (Dicentrarchus labrax) raised against live ...
متن کاملSurvival against exogenous hydrogen peroxide of Photobacterium damselae subsp. piscicida under different culture conditions.
Photobacterium damselae subsp. piscicida is a fish pathogen responsible for important losses in aquaculture world-wide. Several studies on its virulence mechanisms have been carried out and outer membrane proteins involved in the acquisition of iron or production of extracellular products have been suggested as the main determinants of its virulence for fish (Magariños, Santos, Romalde, Rivas, ...
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The taxonomic position of Photobacterium damselae subsp. piscicida, the causative agent of fish pasteurellosis, is controversial as this organism has also been described as 'Pasteurella piscicida'. To clarify the taxonomic position of the pathogen, a total of 113 P. damselae subsp. piscicida strains and 20 P. damselae subsp. damselae strains, isolated from different geographical areas and from ...
متن کاملIn vivo morphological and antigenic characteristics of Photobacterium damselae subsp. piscicida
The present study was conducted to examine the morphology and antigenicity of Photobacterium damselae subsp. piscicida by culturing the bacterium in vivo in the peritoneal cavity of sea bass (Dicentrarchus labrax) within dialysis bags with either a low molecular weight (LMW) cut-off of 25 kDa or a high molecular weight (HMW) cut-off of 300 kDa. Differences were observed in the growth rate betwe...
متن کاملSuperoxide dismutase and catalase activities in Photobacterium damselae ssp. piscicida.
The ability of a set of Photobacterium damselae ssp. piscicida strains isolated from different fish species to produce different superoxide dismutase (SOD) and catalase enzymes was determined. Unlike other bacterial pathogens, P. damselae ssp. piscicida is not able to produce different isoforms of SOD or catalase containing different metal cofactors when cultured under oxidative stress induced ...
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ژورنال
عنوان ژورنال: Journal of Veterinary Science
سال: 2007
ISSN: 1229-845X
DOI: 10.4142/jvs.2007.8.3.255